Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

(-)-Grandisin form Cryptocarya crassinervia.

J M Saad1, E Soepadamo, X P Fang

  • 1Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.

Journal of Natural Products
|November 1, 1991
PubMed
Summary

The lignan (-)-grandisin was isolated from Cryptocarya crassinervia using the brine shrimp lethality test. This compound showed no significant cytotoxicity against human tumor cells in preliminary tests.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of substituting wet corn gluten feed and corn stover for alfalfa hay in total mixed ration silage on lactation performance in dairy cows.

Animal : an international journal of animal bioscience·2021
Same author

Effects of replacing soybean meal with pumpkin seed cake and dried distillers grains with solubles on milk performance and antioxidant functions in dairy cows.

Animal : an international journal of animal bioscience·2021
Same author

First Report of Clubroot on Capsella bursa-pastoris Caused by Plasmodiophora brassicae in Sichuan Province of China.

Plant disease·2019
Same author

Reductive silylation of Cp*UO2((Mes)PDI(Me)) promoted by Lewis bases.

Dalton transactions (Cambridge, England : 2003)·2016
Same author

Bioactive flavonoids from the black locust tree, robinia pseudoacacia.

Pharmaceutical biology·2011
Same author

Focus-position sensing using laser speckle.

Applied optics·2010

Area of Science:

  • Natural Product Chemistry
  • Pharmacognosy
  • Organic Chemistry

Background:

  • Cryptocarya crassinervia is a plant source of bioactive compounds.
  • Lignans are a class of natural products with diverse biological activities.

Purpose of the Study:

  • To isolate and characterize the lignan (-)-grandisin from Cryptocarya crassinervia.
  • To evaluate the cytotoxic potential of (-)-grandisin against human tumor cells.

Main Methods:

  • Brine shrimp lethality test for bioassay-guided isolation.
  • Spectroscopic techniques including IR, 1H NMR, and MS for structural elucidation.
  • X-ray crystallography for determining relative stereochemistry.

Main Results:

Related Experiment Videos

  • The known lignan (-)-grandisin was successfully isolated and identified.
  • The structure was confirmed as an all-trans α,α'-diaryl-β,β'-dimethyltetrahydrofuran.
  • Compound 1 exhibited no significant cytotoxicity in a panel of human tumor cell lines.

    • Conclusions:

    • (-)-Grandisin is a lignan present in Cryptocarya crassinervia.
    • The compound's cytotoxic activity against tested human tumor cells is negligible.
    • Further research may explore other biological activities of (-)-grandisin.