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Cellular components of proliferative vitreoretinal membranes
1Department of Ophthalmology, College of Medicine, Seoul National University, Korea.
Korean Journal of Ophthalmology : KJO
|December 1, 1991
Summary
Identifying cellular components of proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR) membranes is key to understanding pathogenesis. PVR membranes primarily contain retinal pigment epithelial cells and fibroblasts, while PDR membranes consist mainly of mesenchymal cells.
Area of Science:
- Ophthalmology
- Cell Biology
- Pathogenesis Research
Background:
- Proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR) involve the formation of cellular membranes.
- Accurate identification of cellular components is crucial for understanding the pathogenesis of these membranes.
Purpose of the Study:
- To identify the cellular components of proliferative vitreoretinal membranes in PVR and PDR.
- To elucidate the cellular basis of membrane formation in these retinal diseases.
Main Methods:
- Immunohistochemical techniques were employed to analyze surgically obtained membranes.
- Monoclonal antibodies against cytokeratin, glial fibrillary acidic protein (GFAP), and vimentin were used.
- The immunoperoxidase technique (ABC method) was applied for staining.
Main Results:
- In PVR membranes, cell positivity was 48% for cytokeratin, 1% for GFAP, and 92% for vimentin.
- In PDR membranes, cell positivity was 0% for cytokeratin, 5% for GFAP, and 93% for vimentin.
- Retinal pigment epithelial cells and fibroblasts are major components of PVR membranes.
Conclusions:
- PVR membranes are predominantly composed of retinal pigment epithelial cells and fibroblasts.
- Mesenchymal cells are the major cellular components of PDR membranes, with glial cells being minor components.
- These findings contribute to understanding the pathogenesis of proliferative vitreoretinal diseases.