Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Do evolutionary changes in cytochrome c structure reflect functional adaptations?

E Margoliash, S Ferguson-Miller, C H Kang

    Federation Proceedings
    |August 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effectiveness of transarterial embolisation for intractable postpartum haemorrhage in a disseminated intravascular coagulation state, despite emergency hysterectomy.

    Clinical radiology·2022
    Same author

    Asymptomatic Cerebral Vasoconstriction after Carotid Artery Stenting.

    AJNR. American journal of neuroradiology·2020
    Same author

    Tuberculosis infection and lung adenocarcinoma: Mendelian randomization and pathway analysis of genome-wide association study data from never-smoking Asian women.

    Genomics·2019
    Same author

    Lysolecithin as feed additive enhances collagen expression and villus length in the jejunum of broiler chickens.

    Poultry science·2017
    Same author

    Use of Extracorporeal Membrane Oxygenation Prior to Lung Transplantation Does Not Jeopardize Short-term Survival.

    Transplantation proceedings·2015
    Same author

    Inguinal Hernia: Recurrences, Tailored Surgery & Pubic Inguinal Pain Syndrome (Sportsman Hernia).

    Hernia : the journal of hernias and abdominal wall surgery·2015
    Same journal

    ASBESTOS FIBERS MEDIATE THE UPTAKE OF DNA INTO PRIMATE CELLS IN CULTURE.

    Federation proceedings·2018
    Same journal

    Chemical protection of mammalian tissues.

    Federation proceedings·2014
    Same journal

    Dietary requirements for fertility and lactation; dried yeasts as sources of proteins and vitamin B complex for growth, reproduction and lactation.

    Federation proceedings·2010
    Same journal

    On the mode of action of chlorinating compounds.

    Federation proceedings·2010
    Same journal

    d-Amino acid oxidase of Proteus morganii.

    Federation proceedings·2010
    Same journal

    Studies on thymus nucleohistone.

    Federation proceedings·2010
    See all related articles

    Evolutionary changes in cytochrome c are not constant, challenging neutral mutation theory. A high-affinity reaction with cytochrome c oxidase reveals species-specific differences, suggesting a role in mitochondrial respiratory control.

    Area of Science:

    • Biochemistry
    • Evolutionary Biology
    • Molecular Biology

    Background:

    • The rate of evolutionary change in eukaryotic cytochromes c amino acid sequences is variable, questioning the dominance of neutral mutations.
    • Previous research indicated functional indistinguishability of eukaryotic cytochromes c in mitochondrial respiratory chain interactions.

    Purpose of the Study:

    • To investigate potential functional differences in cytochrome c from various species.
    • To explore the kinetics of cytochrome c interaction with cytochrome c oxidase.
    • To assess the role of nucleotides in modulating this interaction and its implications for mitochondrial respiratory control.

    Main Methods:

    • Kinetic analysis of cytochrome c reactions with cytochrome c oxidase at low ionic strength.
    • Direct binding studies to determine dissociation constants.

    Related Experiment Videos

  • Investigation of inhibition by ADP and ATP at physiological concentrations.

    • Main Results:

    • A high-affinity reaction between cytochrome c and cytochrome c oxidase was discovered, with significant activity differences among species (horse, yeast, Euglena).
    • Observed Km values (10^-7 to 10^-8 M) correlate with dissociation constants from binding studies.
    • The reaction is sensitive to ionic strength and inhibited by ADP and ATP, with ATP being more potent.

    Conclusions:

    • Functional differences in cytochrome c exist, contradicting previous assumptions of indistinguishability.
    • The ATP-mediated inhibition of cytochrome c binding to its oxidase suggests a novel mechanism for mitochondrial respiratory control.
    • These findings open avenues for studying evolutionary adaptations of cytochromes c in relation to their oxidases.