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Updated: Aug 6, 2026

Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Published on: July 12, 2015
[Transitional mucosa in carcinoma of large bowel. Mucin histochemical and scanning electron microscopical study]
1Xing qiao Hospital, Third Military Medical college, Chongqing.
Abstract:
We studied the mucosa close to the carcinoma of large bowel (CLB) in 200 cases, the carcinoma tissue of CLB in 130 and the mucosa of fetus large bowel in 24 by mucin histochemistry, in addition to the mucosa near CLB studied by scanning electron microscope (SEM) in 25. The results suggested that mucin composition of transitional mucosa (TM) was similar to CLB and large bowel mucosa of the fetus. SEM showed significant changes in TM. The HID/AB Stain suggesting abnormal range of TM was within 2 cm in 68.0% (136/200) of cases of CLB, within 3 cm in 88.5% (177/200) and In SEM abnormal range within 2 cm in 88.0% (22/25). The measurement of TM can provide reference for resectable range of CLB.
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