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Related Experiment Videos

Intermittent-flow versus continuous-flow mononuclear cell apheresis.

J Storek1, J Nepomucka, P Kobylka

  • 1Institute of Hematology and Blood Transfusion, Prague, Czechoslovakia.

Haematologia
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

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The continuous-flow Fenwall CS-3000 is better for collecting mononuclear cells (MNCs) than the intermittent-flow Haemonetics V-50. While MNC yield was similar, the CS-3000 had less contamination.

Area of Science:

  • Hematology
  • Biomedical Engineering
  • Cellular Biology

Background:

  • Blood cell separators are crucial for isolating specific cell populations.
  • Intermittent-flow and continuous-flow systems represent different technological approaches to apheresis.
  • Optimizing mononuclear cell (MNC) collection is vital for various clinical and research applications.

Purpose of the Study:

  • To compare the efficacy of an intermittent-flow (Haemonetics V-50) and a continuous-flow (Fenwall CS-3000) blood cell separator for mononuclear cell (MNC) collection.
  • To evaluate differences in cell yield and purity between the two apheresis systems.

Main Methods:

  • A comparative study involving 17 aphereses using the V-50 and 24 aphereses using the CS-3000 on healthy donors.
  • Analysis of mononuclear cell (MNC) yield and colony-forming unit-granulocyte-macrophage (CFU-GM) yield.

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  • Assessment of contamination by red blood cells, polymorphonuclear cells, and platelets.

    • Main Results:

    • No significant difference was observed in MNC yield (V-50: median 6.4 x 10^9; CS-3000: median 6.3 x 10^9).
    • No significant difference was found in CFU-GM yield (V-50: median 41 x 10^3; CS-3000: median 43 x 10^3).
    • The CS-3000 system demonstrated substantially lower red blood cell and polymorphonuclear cell contamination compared to the V-50 system, with slightly lower platelet contamination.

    Conclusions:

    • The continuous-flow apheresis system (CS-3000) is more suitable for mononuclear cell (MNC) harvesting than the intermittent-flow system (V-50).
    • The CS-3000 offers a purer MNC product, which is advantageous for downstream applications.
    • System design impacts the efficiency and purity of cell separation in apheresis procedures.