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Stability of cefotaxime sodium and metronidazole in an i.v. admixture at 8 degrees C
T E Rivers1, H A McBride, J M Trang
1Department of Pharmacy, Baptist Medical Center Princeton, Birmingham, AL 35211.
Abstract:
The stability of cefotaxime and metronidazole in i.v. admixture at 8 degrees C was studied. The commercially available injectable formulation of cefotaxime sodium 1 g was diluted to 5 mL with 0.9% sodium chloride injection and added to metronidazole injection 500 mg/100 mL. A 2-mL sample was removed and diluted to 100 mL with water. Thirty 1-mL portions were transferred to glass vials and refrigerated at 8 degrees C. At 0, 1, 2, 4, 8, 12, 24, 36, 48, and 72 hours after the admixture was prepared, the vials were removed, placed in a refrigerated autosampler, and assayed for cefotaxime and metronidazole concentrations by stability-indicating high-performance liquid chromatography. Over the 72-hour study period, the concentration of cefotaxime remaining at all assay times was 95.91-101.13% of the initial concentration. The concentration of metronidazole remaining at each assay time was 93.08-102.19% of the initial concentration. Cefotaxime sodium 10 mg/mL and metronidazole 5 mg/mL were stable for 72 hours at 8 degrees C in an i.v. admixture prepared from commercially available injectable formulations.
Insights
Cefotaxime sodium and metronidazole intravenous admixtures demonstrate excellent stability for up to 72 hours at 8 degrees C. This finding supports the safe co-administration of these critical antibiotics in clinical settings.
Area of Science:
- Pharmaceutical Sciences
- Clinical Pharmacy
- Drug Stability
Background:
- Cefotaxime and metronidazole are frequently co-administered antibiotics.
- Assessing the stability of drug admixtures is crucial for safe intravenous therapy.
Purpose of the Study:
- To evaluate the stability of cefotaxime and metronidazole when combined in an intravenous admixture.
- To determine the compatibility and degradation profile of the antibiotic combination over time.
Main Methods:
- Commercially available cefotaxime sodium and metronidazole injections were prepared as an admixture.
- Samples were stored at 8 degrees C and analyzed at various time points up to 72 hours.
- High-performance liquid chromatography (HPLC) was used to quantify drug concentrations.
Main Results:
- Cefotaxime concentrations remained between 95.91% and 101.13% of the initial concentration over 72 hours.
- Metronidazole concentrations ranged from 93.08% to 102.19% of the initial concentration throughout the study.
- Both drugs exhibited high stability in the admixture under refrigerated conditions.
Conclusions:
- Cefotaxime sodium and metronidazole are stable in intravenous admixtures for 72 hours at 8 degrees C.
- The study supports the clinical use of this cefotaxime-metronidazole admixture, ensuring therapeutic efficacy.
- Refrigerated storage at 8 degrees C is suitable for maintaining the integrity of this antibiotic combination.