Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sterilization beneath rings on dental instruments.

C H Miller1, M A Sheldrake

  • 1Department of Oral Microbiology, Indiana University School of Dentistry, Indianapolis 46202.

American Journal of Dentistry
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of trauma centre capacity and volume on the mortality risk of incoming new admissions.

BMJ military health·2020
Same author

Mathematical model of thrombin generation and bleeding phenotype in Amish carriers of Factor IX:C deficiency vs. controls.

Thrombosis research·2019
Same author

Distinguishing lupus anticoagulants from factor VIII inhibitors in haemophilic and non-haemophilic patients.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same author

Reagent substitutions in the Centers for Disease Control and Prevention Nijmegen-Bethesda assay for factor VIII inhibitors.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same author

Effects of pre-analytical heat treatment in factor VIII (FVIII) inhibitor assays on FVIII antibody levels.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same author

Laboratory testing for factor VIII and IX inhibitors in haemophilia: A review.

Haemophilia : the official journal of the World Federation of Hemophilia·2018

Instrument rings can hinder sterilization effectiveness. Steam autoclaves and unsaturated chemical vapor sterilizers offer the greatest reliability for sterilizing instruments with rings.

Area of Science:

  • Dental instrument reprocessing
  • Sterilization validation
  • Microbial contamination control

Background:

  • Instrument rings are common in dental settings.
  • Potential for microbial contamination beneath instrument rings is a concern.
  • Standard sterilization methods may be compromised by instrument accessories.

Purpose of the Study:

  • To evaluate the efficacy of common sterilization methods for instruments with attached rings.
  • To determine if instrument rings impede effective sterilization.
  • To identify optimal sterilization parameters for ringed dental instruments.

Main Methods:

  • Dental instruments were contaminated with bacterial spores (Bacillus stearothermophilus/subtilis).
  • Instrument rings were placed over contamination sites.

Related Experiment Videos

  • Instruments were processed using steam autoclave, unsaturated chemical vapor, dry heat, ethylene oxide, or glutaraldehyde solution.
  • Post-sterilization cultures assessed for viable spores on instruments and rings.

    • Main Results:

    • Sterilization effectiveness varied significantly between methods.
    • Steam autoclaving and unsaturated chemical vapor sterilization demonstrated the highest reliability for ringed instruments.
    • Dry heat, ethylene oxide, and glutaraldehyde showed reduced efficacy in sterilizing beneath instrument rings.

    Conclusions:

    • Instrument rings can create challenges for complete sterilization.
    • Steam autoclave and unsaturated chemical vapor sterilization are recommended for instruments with rings.
    • Careful consideration of sterilization methods is crucial when using instrument accessories.