Muscle denervation increases thyrotropin-releasing hormone (TRH) biosynthesis in the rat medullary raphe

To determine whether thyrotropin-releasing hormone (TRH) could exert a trophic role in ventral horn motor neurons, we examined the effect of muscle denervation with botulinum toxin A on TRH mRNA in the rat medullary raphe by in situ hybridization histochemistry. Compared to controls, denervated rats showed a significant increase in the number and silver grain density of hybridized medullary raphe neurons. Increased proTRH gene expression in the medullary raphe in response to motor unit perturbation indicates that TRH may be trophic to lower motor neurons.