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Class II MHC antigen expression by cultured human cerebral vascular endothelial cells
R M McCarron1, L Wang, E P Cowan
1Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Brain Research
|December 6, 1991
Abstract:
Cerebral vascular endothelial cells (EC) isolated from human brain do not constitutively express class II MHC antigens. However, incubation in the presence of human interferon-gamma (IFN gamma) resulted in the expression of both HLA-DR and -DP antigens. FACS analysis revealed that approximately 40% of the EC population expressed HLA-DR antigen. Quantitation by ELISA demonstrated that maximum expression was observed with 100 U/ml IFN gamma for 4 days. Treatment with IFN gamma also increased class II mRNA levels in all EC cultures tested.