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Related Experiment Video

Updated: Jul 8, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

A mouse and embryonic stem cell derived from a single embryo.

Takeshi Teramura¹, Toshiyuki Takehara, Naoko Kishi

¹Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, Tsu, Mie, Japan.

Cloning and Stem Cells

|December 25, 2007

Summary

Researchers efficiently created embryonic stem cells (ESCs) and pups from single embryos. This method allows for developmental studies and genetic modifications without embryo destruction, advancing stem cell research.

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Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

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Last Updated: Jul 8, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

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Published on: August 20, 2017

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Area of Science:

Developmental Biology
Stem Cell Biology
Reproductive Biology

Background:

Embryonic stem cells (ESCs) are valuable for studying mammalian development, genetic modification, and drug discovery due to their self-renewal and differentiation capabilities.
Ethical concerns surrounding human embryo use for ESC derivation have spurred research into alternative methods.
Previous studies have derived ESCs from biopsied blastomeres, but producing both an ESC and a viable offspring from a single embryo remained unachieved.

Purpose of the Study:

To develop an efficient method for deriving both embryonic stem cells (ESCs) and viable offspring from individual mammalian embryos.
To demonstrate that blastomeres from early-stage embryos retain pluripotency and viability for dual derivation.
To establish a less ethically contentious approach for generating ESCs for research and therapeutic applications.

Main Methods:

Embryos at the two-cell stage were subjected to blastomere biopsy.
Individual blastomeres were cultured to derive embryonic stem cells (ESCs).
The remaining blastomeres were cultured to assess developmental potential towards producing an offspring.

Main Results:

The study successfully produced pairs of ESCs and live pups from individual embryos in 3 out of 20 attempts, demonstrating efficiency.
Blastomere-derived ESCs exhibited multilineage differentiation potential and contributed to chimeric mice.
Separated blastomeres from the two-cell stage were shown to maintain viability for developing into either ESCs or a complete organism.

Conclusions:

Each blastomere at the two-cell stage possesses full pluripotency and the capacity for independent development into an ESC or a pup.
This dual derivation method offers an efficient and ethically considerate approach to generating ESCs and genetically modified animals.
The findings support the potential of blastomere-derived ESCs for applications in developmental biology, regenerative medicine, and drug discovery.