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Centrifugation

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Two-column simulated moving-bed process for binary separation.

Rui C R Rodrigues¹, Tiago J S B Canhoto, João M M Araújo

¹Requimte/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.

Journal of Chromatography. A

|December 25, 2007

Summary

This summary is machine-generated.

A novel two-column simulated moving-bed system enhances binary separation efficiency. This chromatography method offers improved performance over traditional batch systems for separating nucleosides.

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Area of Science:

Chemical Engineering
Separation Science
Chromatography

Background:

Simulated moving-bed (SMB) chromatography is a powerful technique for continuous binary separation.
Optimizing SMB systems is crucial for improving efficiency and reducing operational costs.
Existing batch chromatography methods with recycling can be less efficient for complex separations.

Purpose of the Study:

To develop and demonstrate a novel two-column simulated moving-bed (SMB) system for binary separation.
To evaluate the performance of the two-column SMB system compared to single-column batch chromatography with recycling.
To investigate the impact of separation difficulty and desorbent-to-feed ratios on the system's performance.

Main Methods:

Development of a two-column SMB system featuring flexible node design, robust pump configuration, and cyclic flow-rate modulation.
Demonstration of the system's feasibility using reversed-phase chromatography for the linear separation of two nucleosides.
Performance verification through laboratory-scale experiments and detailed process simulations.

Main Results:

The two-column SMB system successfully achieved binary separation of nucleosides.
The SMB system demonstrated potential advantages over single-column batch chromatography with recycling.
Simulations and experiments confirmed the system's effectiveness across varying separation difficulties and desorbent-to-feed ratios.

Conclusions:

The developed two-column SMB system is a viable and potentially superior alternative for binary separations.
The system's design and operational flexibility allow for optimization in challenging chromatographic applications.
This advancement in SMB technology offers enhanced efficiency for nucleoside separation and other binary mixtures.