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Updated: Jul 8, 2026

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Published on: February 17, 2016
Cholinergic regulation of neuropeptide Y synthesis and release in human neuroblastoma cells
Elena Dozio1, Massimiliano Ruscica, Daniel Feltrin
1Istituto di Endocrinologia, Center of Excellence on Neurodegenerative Diseases and Center for Endocrinological Oncology, University of Milan, Milano, Italy.
Abstract:
The biosynthesis and release of neuropeptide Y (NPY) is regulated by several factors. Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). In conclusion, the present observations support the hypothesis that muscarinic receptor activation regulates the biosynthesis and secretion of NPY.
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