Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Note: multiplexed multiple-tau auto- and cross-correlators on a single field programmable gate array.

The Review of scientific instruments·2012
Same author

Protein disorder prevails under crowded conditions.

Biochemistry·2011
Same author

Brownian dynamics simulation of DNA unrolling from the nucleosome.

The journal of physical chemistry. B·2009
Same author

Escherichia coli low-copy-number plasmid R1 centromere parC forms a U-shaped complex with its binding protein ParR.

Nucleic acids research·2007
Same author

DNA damaging capability of hematoporphyrin towards DNAs of various accessibilities.

Journal of photochemistry and photobiology. B, Biology·2006
Same author

Polymer chain models of DNA and chromatin.

The European physical journal. E, Soft matter·2006

Related Experiment Video

Updated: Jul 8, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Protein-protein interactions determined by fluorescence correlation spectroscopy.

J Langowski1

  • 1German Cancer Research Center, Division Biophysics of Macromolecules, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany.

Methods in Cell Biology
|December 25, 2007
PubMed
Summary
This summary is machine-generated.

Fluorescence correlation spectroscopy (FCS) detects biomolecular interactions via correlated motion. This sensitive technique measures interactions, concentrations, and mobilities in living cells using autofluorescent proteins.

More Related Videos

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Related Experiment Videos

Last Updated: Jul 8, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Area of Science:

  • Biophysics
  • Cell Biology
  • Biochemistry

Background:

  • Fluorescence correlation spectroscopy (FCS) is an advanced biophysical technique.
  • It detects interactions between biomolecules by analyzing their correlated motion.

Purpose of the Study:

  • Introduce the principles of Fluorescence Correlation Spectroscopy (FCS).
  • Review recent applications of FCS in living cell research.

Main Methods:

  • Utilizes the correlated motion of fluorescently labeled biomolecules.
  • Employs autofluorescent proteins for detection within living cells.

Main Results:

  • Demonstrates high sensitivity, capable of single-molecule detection.
  • Provides orientation-independent and distance-independent measurements.
  • Enables simultaneous assessment of molecular interactions, concentrations, and mobilities.

Conclusions:

  • FCS is a powerful tool for studying protein-protein interactions in vivo.
  • Autofluorescent proteins facilitate FCS measurements of molecular dynamics in living cells.