Related Experiment Videos
Bacterial leakage in root canals obturated by different techniques. Part 1: microbiologic evaluation
Viviane Haiub Brosco1, Norberti Bernardineli, Sérgio Aparecido Torres
1Department of Endodontics, Bauru Dental School, University of São Paulo, Bauru, Brazil. vivianebrosco@yahoo.com.br
Summary
Tagger's hybrid technique resulted in more coronal leakage in root canals compared to other methods. Obturating root canals to 10 mm significantly reduced leakage compared to 5 mm obturation.
Area of Science:
- Endodontics
- Microbiology
- Dental Materials
Background:
- Coronal leakage is a significant factor in endodontic treatment failure.
- Effective root canal obturation is crucial for preventing bacterial ingress.
- Various obturation techniques and depths are employed, each with potential implications for seal quality.
Purpose of the Study:
- To compare the coronal bacterial leakage of root canals obturated with different techniques.
- To evaluate the effect of obturation length (5 mm vs. 10 mm) on coronal leakage.
Main Methods:
- 160 palatal roots of maxillary molars were instrumented.
- Roots were obturated using lateral condensation, Microseal, Touch 'n Heat + Ultrafil, or Tagger's hybrid technique at 5 mm or 10 mm depths.
- Roots were sterilized and subjected to bacterial leakage evaluation.
Main Results:
- Tagger's hybrid technique showed a statistically significant increase in coronal leakage compared to other methods.
- No significant difference in leakage was observed between lateral condensation, Microseal, and Touch 'n Heat + Ultrafil techniques.
- Obturation to 10 mm resulted in significantly less coronal leakage than obturation to 5 mm.
Conclusions:
- Tagger's hybrid technique may compromise coronal seal integrity.
- Deeper obturation (10 mm) provides a superior coronal seal compared to shallower obturation (5 mm).
- Careful selection of obturation technique and depth is essential for predictable endodontic outcomes.
Related Concept Videos
Key Techniques in Microbiology
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...