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Determination of dehydroalanine residues in proteins and peptides: an improved method
N A Bartone1, J D Bentley, J A Maclaren
1CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia.
Journal of Protein Chemistry
|December 1, 1991
Abstract:
Dehydroalanine residues in peptides and proteins react with 4-pyridoethanethiol in alkaline solution to form 4-pyridoethyl cysteine residues, which after acid hydrolysis, give the corresponding amino acid. An experimental protocol has been developed, and with simple dehydroalanine derivatives, conversions of 96-99% are achieved. Tests with peptides and proteins show that serine and cysteine/cystine residues do not interfere with this analytical procedure. A sample of wool keratin contained 57 mumol dehydroalanine/g.