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Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Introduction to Actin01:26

Introduction to Actin

Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across different species.
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Non-nuclear Inheritance01:29

Non-nuclear Inheritance

Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm⁠—such as chloroplasts and mitochondria⁠—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
The Phragmoplast01:59

The Phragmoplast

Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...

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Related Experiment Video

Updated: Jul 8, 2026

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
06:28

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Published on: May 16, 2025

Nuclear actin in plants.

José R Cruz1, Consuelo de la Torre, Susana Moreno Díaz de la Espina

  • 1Centro Investigaciones Biológicas, CSIC Ramiro de Maeztu 9, Madrid 28040, Spain.

Cell Biology International
|December 25, 2007
PubMed
Summary

Plant nuclei contain actin proteins and myosin I beta (NMI), previously thought absent in plants. These proteins are integral to transcription complexes and the nucleoskeleton, challenging prior biological assumptions.

Area of Science:

  • Plant cell biology
  • Molecular biology
  • Cytoskeletal research

Background:

  • Actins are key cytoskeletal proteins in animal cells, with nuclear forms involved in complexes and myosin I beta (NMI) interactions.
  • Nuclear actin-related proteins (ARPs) and nuclear actin-binding proteins (nABPs) are known in animals, but nuclear actin was considered absent in plants.
  • Plants were believed to lack orthologues of major animal structural nABPs, suggesting a different nuclear architecture.

Purpose of the Study:

  • To investigate the presence and function of actin and myosin I beta (NMI) in the plant nucleus.
  • To determine if plant nuclear actin is involved in transcription complexes and the nucleoskeleton.
  • To challenge the established view of nuclear actin absence in plants.

Main Methods:

More Related Videos

A Streamlined Protocol for Single-Molecule Localization Microscopy in Arabidopsis Nuclei
09:54

A Streamlined Protocol for Single-Molecule Localization Microscopy in Arabidopsis Nuclei

Published on: May 8, 2026

Related Experiment Videos

Last Updated: Jul 8, 2026

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
06:28

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Published on: May 16, 2025

A Streamlined Protocol for Single-Molecule Localization Microscopy in Arabidopsis Nuclei
09:54

A Streamlined Protocol for Single-Molecule Localization Microscopy in Arabidopsis Nuclei

Published on: May 8, 2026

  • Western blotting (WB) to detect actin and NMI proteins in plant nuclear extracts.
  • Confocal immunofluorescence microscopy using antibodies against human actin and NMI to visualize their localization within plant nuclei.
  • Analysis of actin solubility to characterize different forms present in the nucleus.
  • Main Results:

    • Evidence for the presence of actin forms with varying solubility in the plant nucleus.
    • Detection of the associated protein myosin I beta (NMI) within the plant nucleus.
    • Localization of nuclear actin and NMI as components of transcription complexes and the nucleoskeleton.

    Conclusions:

    • Actin and myosin I beta (NMI) are present in the plant nucleus, contrary to previous assumptions.
    • These proteins play roles in plant nuclear organization, specifically in transcription complexes and the nucleoskeleton.
    • This finding necessitates a re-evaluation of nuclear actin's role across different kingdoms of life.