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Updated: Jul 8, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Comparison of 3 automated immunoassays for hepatitis B surface antigen.]
Soo Jin Yoo1, Hye Jeon Oh, Bo Moon Shin
1Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea. bmshin@unitel.co.kr.
Background:
Hepatitis B surface antigen (HBsAg) is one of the most important serological markers used to diagnose hepatitis B virus (HBV) infection. Automated immunoassays have been developed, meeting the current clinical requirement of HBsAg assays over the years. This study was performed to determine the degree of agreements between 3 kinds of HBsAg assay systems.
Methods:
Serum samples from 425 patients were assayed by the HBsAg assay systems of Elecsys (Roche Diagnostics, Germany), ADVIA Centaur (Bayer Diagnostics, USA), and AxSYM (Abbott Laboratories, USA).
Results:
The concordance rates among the 3 assays were 100%. A total of 249 (58.6%) specimens were positive, and their index values showed a weak correlation between the 3 assays; nevertheless, positive specimens with low levels (<10) of index values in one system also presented low values in other systems, and all of them were confirmed by neutralization assays.
Conclusions:
The 3 automated HBsAg assay systems presented a high level of concordance.
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