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[Establishment of reference values for platelet activation markers by flow cytometry.]
1Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea. limyoung@ajou.ac.kr.
The Korean Journal of Laboratory Medicine
|December 25, 2007
Summary
This study establishes reference values for platelet activation markers and leukocyte-platelet aggregates using flow cytometry. These findings aid in interpreting platelet function tests for better clinical evaluation.
Area of Science:
- Hematology
- Immunology
- Flow Cytometry
Context:
- Platelet function tests are crucial for diagnosing and managing various hemostatic disorders.
- Flow cytometry offers a sensitive method for analyzing platelet activation and aggregates.
- Establishing reliable reference values is essential for accurate interpretation of these tests.
Purpose:
- To determine reference values for platelet activation markers (PAC1, CD62P) in resting and activated states.
- To establish reference values for leukocyte-platelet aggregates, including granulocyte- and lymphocyte-platelet aggregates.
- To validate the use of flow cytometry for analyzing these parameters in healthy adults.
Summary:
- Reference ranges for PAC1 (0.1-12.5%) and CD62P (0.0-4.7%) were determined at rest.
- Activated states showed significantly higher expression: PAC1 (65.3-93.1%), CD62P (39.0-91.2%) with ADP or TRAP.
- Reference values for total leukocyte-platelet aggregates (2.8-23.6%), granulocyte-platelet aggregates (5.3-34.2%), and lymphocyte-platelet aggregates (4.9-21.6%) were established.
Impact:
- Provides essential reference data for interpreting flow cytometry-based platelet function tests.
- Enhances the diagnostic accuracy of platelet function assays.
- Supports clinical decision-making in hematological conditions involving platelet dysfunction or activation.
