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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jul 8, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

[HBV DNA Quantitation Using Real-time PCR.].

Jeong Heo¹, Won Ook Go, Gwang Ha Kim

¹Department of Internal Medicine, Pusan National University, School of Medicine, Busan, Korea. hhkim@pusan.ac.kr.

The Korean Journal of Laboratory Medicine

|December 25, 2007

Summary

A new real-time PCR method accurately measures hepatitis B virus (HBV) DNA in patient serum across a wide concentration range. This sensitive assay aids in managing chronic HBV infection and monitoring treatment response.

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Last Updated: Jul 8, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

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Published on: December 15, 2023

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

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Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Area of Science:

Molecular Biology
Virology
Clinical Diagnostics

Context:

Accurate quantification of hepatitis B virus (HBV) DNA is crucial for managing chronic HBV infection and assessing treatment efficacy.
HBV DNA concentrations in patient serum exhibit a broad dynamic range, necessitating sensitive detection methods.
Real-time PCR offers high sensitivity for detecting HBV DNA across various concentrations.

Purpose:

To develop and validate novel primers and probes for a sensitive real-time PCR assay to quantify HBV DNA.
To evaluate the performance characteristics of the new assay, including sensitivity, dynamic range, and precision.
To assess the correlation of the developed real-time PCR assay with a commercial assay for HBV DNA measurement.

Summary:

A new real-time PCR assay was designed using specific primers and probes for HBV DNA detection.
The assay demonstrated high sensitivity (approx. 6.08 x 10^2 copies/mL) and a wide dynamic range (6.1 x 10^2 to 6.5 x 10^9 copies/mL).
Results showed good reproducibility and correlated well with the Cobas Amplicor HBV Monitor assay.

Impact:

Provides a rapid, sensitive, and accurate in-house method for quantifying serum HBV DNA.
Facilitates improved patient management and therapeutic response evaluation in chronic HBV infection.
Offers a reliable alternative for HBV DNA monitoring in clinical laboratories.