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Updated: Jul 8, 2026

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Published on: January 16, 2018
Heterogeneous rRNA molecules encoded by Streptomyces coelicolor M145 genome are all expressed and assembled into
Hyun-Lee Kim1, Eunkyoung Shin, Hong-Man Kim
1Graduate School of Life Science and Biotechnology, Pochon CHA University, Seongnam, Korea.
Abstract:
The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and five kinds of LSU rRNA. Primer extension analyses revealed that all of the heterogeneous rRNA molecules are expressed and assembled into ribosomes. This finding and the maintenance of the intragenomic variability of rRNA operons imply the existence of functional divergence of rRNA species in this developmentally complex microorganism.
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