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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jul 8, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

The microRNA.org resource: targets and expression.

Doron Betel1, Manda Wilson, Aaron Gabow

  • 1Computational and Systems Biology Center, Memorial Sloan-Kettering Cancer Center, NY, USA.

Nucleic Acids Research
|December 26, 2007
PubMed
Summary
This summary is machine-generated.

MicroRNA.org offers a comprehensive database for microRNA target predictions and expression profiles. This resource aids researchers in exploring microRNA gene regulation and interactions in various species.

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

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Related Experiment Videos

Last Updated: Jul 8, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • Understanding miRNA targets and expression is crucial for biological research.
  • Existing resources may lack comprehensive, up-to-date information.

Purpose of the Study:

  • To present microRNA.org, a centralized resource for miRNA target predictions and expression profiles.
  • To provide tools for exploring miRNA-gene interactions and co-regulation.
  • To facilitate novel discoveries in the field of miRNA gene regulation.

Main Methods:

  • Development of the miRanda algorithm incorporating biological rules and a mammalian miRNA compendium for target prediction.
  • Generation of miRNA expression profiles from extensive sequencing of mammalian tissues and cell lines.
  • Implementation of an improved graphical user interface for data exploration.

Main Results:

  • MicroRNA.org provides extensive miRNA target predictions and expression data.
  • Users can explore gene regulation by individual miRNAs and cooperative effects of multiple miRNAs.
  • The resource includes expression profiles across diverse mammalian tissues and cell types.

Conclusions:

  • MicroRNA.org serves as a valuable, comprehensive resource for miRNA research.
  • The flexible database and software architecture support future updates and discoveries.
  • The platform facilitates functional insights into miRNA-gene interactions across species.