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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
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Three-dimensional cellular microarray for high-throughput toxicology assays.

Moo-Yeal Lee1, R Anand Kumar, Sumitra M Sukumaran

  • 1Solidus Biosciences, Inc., Troy, NY 12180, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 28, 2007
PubMed
Summary
This summary is machine-generated.

A novel miniaturized 3D cell-culture array, the DataChip, enables high-throughput toxicity screening of drug candidates and their metabolites. This technology provides reliable cytotoxicity data at early drug discovery stages.

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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)

Published on: May 10, 2016

Area of Science:

  • Biotechnology
  • Toxicology
  • Drug Discovery

Background:

  • High-throughput screening is crucial for early-stage drug discovery.
  • Assessing drug candidate toxicity and metabolite effects requires efficient methods.
  • Current methods can be limited by throughput and compound availability.

Purpose of the Study:

  • To develop a miniaturized 3D cell-culture array for high-throughput toxicity screening.
  • To evaluate the efficacy of the DataChip in assessing drug candidates and their metabolites.
  • To validate the DataChip's performance against conventional assays.

Main Methods:

  • Development of the Data Analysis Toxicology Assay Chip (DataChip) with 1,080 miniaturized 3D cell cultures.
  • Encapsulation of human cells in collagen or alginate gels (20 nl) on a functionalized glass slide.
  • Integration with the Metabolizing Enzyme Toxicology Assay Chip (MetaChip) for simultaneous analysis of compounds and P450 metabolites (CYP1A2, CYP2D6, CYP3A4).

Main Results:

  • The DataChip successfully provided IC(50) values for nine compounds and their metabolites.
  • Similar cytotoxicity responses were observed between the DataChip and traditional 96-well plate assays.
  • Miniaturization by nearly 2,000-fold did not impact the cytotoxicity response.

Conclusions:

  • The miniaturized DataChip enables efficient, high-throughput toxicity screening of drug candidates and metabolites.
  • This technology is compatible with compound availability in early-stage drug discovery.
  • The DataChip offers a validated, miniaturized alternative to conventional toxicity assays.