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Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...

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Related Experiment Video

Updated: Jul 8, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

An update on newer beta-lactamases.

Varsha Gupta1

  • 1Department of Microbiology, Government Medical College & Hospital, Chandigarh, India. varshagupta_99@yahoo.com

The Indian Journal of Medical Research
|December 28, 2007
PubMed
Summary

Beta-lactamase production is a major cause of antibiotic resistance, with extended-spectrum beta-lactamases (ESBLs) and carbapenemases posing significant global health threats. Early detection and infection control are crucial for managing these resistant bacteria.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Antibiotic resistance, particularly to beta-lactam antibiotics, is a growing global concern.
  • Beta-lactamase production is the primary mechanism driving this resistance.
  • The prevalence of beta-lactamase-producing organisms, including extended-spectrum beta-lactamases (ESBLs) and carbapenemases, is increasing worldwide.

Purpose of the Study:

  • To highlight the increasing problem of beta-lactamase-mediated antibiotic resistance.
  • To discuss the mechanisms and implications of various beta-lactamases, including ESBLs, Amp C, and carbapenemases (MBLs).
  • To emphasize the need for effective detection methods and infection control strategies.

Main Methods:

  • Review of global and Indian figures on beta-lactamase-producing organisms.

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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Related Experiment Videos

Last Updated: Jul 8, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

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  • Discussion of the enzymatic profiles and resistance patterns associated with ESBLs, Amp C beta-lactamases, and carbapenemases.
  • Overview of current therapeutic strategies and the importance of infection control.

    • Main Results:

    • A marked increase in beta-lactamase-producing organisms globally and in India.
    • Emergence and spread of carbapenemases (MBLs) from Pseudomonas aeruginosa to Enterobacteriaceae.
    • ESBL-producing Enterobacteriaceae exhibit resistance to multiple antibiotic classes, often co-transferring resistance genes.

    Conclusions:

    • Beta-lactamase-producing organisms represent a significant challenge, potentially leading to therapeutic dead ends.
    • Early recognition of these enzymes in clinical microbiology laboratories is essential.
    • Effective management requires a combination of appropriate therapies, infection control practices, and barrier precautions.