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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

The cytoplasmic domain of chondrolectin interacts with the beta-subunit of Rab geranylgeranyl transferase

An Claessens1, Christine Weyn, Joseph Merregaert

  • 1Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.

Cellular & Molecular Biology Letters
|December 28, 2007
PubMed

Insights

Mouse chondrolectin (chodl), a transmembrane protein, interacts with Rab geranylgeranyl transferase beta-subunit (Rabggtb). This finding advances understanding of C-type lectin family functions in skeletal muscle, heart, and testes.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Mouse chondrolectin (chodl) is the mouse homolog of human CHODL, a type Ia transmembrane protein.
  • CHODL is expressed in human testis, prostate, heart, and skeletal muscle, with splice variants found in leukocytes.
  • Chodl is predominantly expressed in adult mouse skeletal muscle, heart, testes, and lungs, and in embryonic tissues.

Purpose of the Study:

  • To investigate the function of mouse chondrolectin (chodl) by identifying its protein interaction partners.
  • To explore the biological roles of C-type lectin family members.

Main Methods:

  • PCR-based subtractive cDNA library screening was used to isolate chodl.
  • A complete Sos recruitment system (SRS) screen with the cytoplasmic domain of chodl was performed to identify interaction partners.
  • In vitro transcription/translation and co-immunoprecipitation (co-IP) experiments were used to confirm protein interactions.

Main Results:

  • The beta-subunit of Rab geranylgeranyl transferase (Rabggtb) was identified as a binding partner of chodl.
  • Rabggtb was isolated multiple times during the SRS screen, indicating a strong interaction.
  • The interaction between chodl and Rabggtb was validated through in vitro and co-IP experiments.

Conclusions:

  • Mouse chondrolectin (chodl) interacts with Rab geranylgeranyl transferase beta-subunit (Rabggtb).
  • This interaction provides insights into the functional roles of chondrolectin family proteins.
  • Further research into chodl-Rabggtb interactions may elucidate their roles in skeletal muscle, heart, and testes biology.

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