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Tissue level lymphocyte subpopulations in donovanosis.
V N Sehgal1, M M Gupta, V K Jain
1Department of Dermatology and Venereology, Maulana Azad Medical College, New Delhi, India.
International Journal of Dermatology
|December 1, 1991
Summary
This study found ulcerogranulomatous donovanosis has a stronger cell-mediated immune response than hypertrophic donovanosis, indicated by a higher T4:T8 lymphocyte ratio and fewer Donovan bodies in tissue. This suggests differences in immune activity between donovanosis variants.
Area of Science:
- Immunology
- Dermatology
- Infectious Diseases
Background:
- Donovanosis, a chronic infectious disease, presents with various clinical forms.
- Understanding the immune response in different donovanosis variants is crucial for effective management.
Purpose of the Study:
- To compare the immune cell populations, specifically T-lymphocytes, in different clinical variants of donovanosis.
- To investigate the relationship between lymphocyte profiles and the presence of Donovan bodies.
Main Methods:
- Immunohistochemistry (PAP technique) was used to quantify T- and B-lymphocyte populations in tissue sections from 22 donovanosis patients.
- Monoclonal antibodies were employed to identify T4 and T8 lymphocyte subsets.
- Tissue smears and Giemsa-stained sections were examined for the presence of Donovan bodies.
Main Results:
- T- and B-lymphocyte infiltrations were similar in ulcerogranulomatous and hypertrophic donovanosis variants.
- The T4:T8 lymphocyte ratio was significantly higher in ulcerogranulomatous (1.41) compared to hypertrophic (1.28) variants.
- Fewer Donovan bodies were observed in tissue smears and sections of the ulcerogranulomatous variant.
Conclusions:
- Ulcerogranulomatous donovanosis exhibits a more pronounced cell-mediated immune response compared to the hypertrophic variant.
- The observed differences in immune response may correlate with the lower bacterial load in ulcerogranulomatous lesions.