How to achieve sustained and complete protein release from PLGA-based microparticles?

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Protein Chemistry

Background:

• Sustained and complete release of therapeutic proteins from poly(lactic-co-glycolic acid) (PLGA) microparticles is a significant challenge in drug delivery.
• Incomplete protein release and destabilization are complex issues often evaluated through in vitro studies.

Purpose of the Study:

• To review strategies for overcoming protein destabilization during release from PLGA microparticles.
• To provide insights into protecting therapeutic proteins within microparticle formulations.

Main Methods:

• Literature review of existing strategies for protein stabilization in PLGA microparticles.
• Analysis of methods involving stabilizers, protein modification, polymer modification, and environmental control.

Main Results:

• Proteins can be protected by incorporating stabilizers into the formulation.
• Protein or polymer modification can enhance protein stability.
• Strategies exist to prevent the formation of destabilizing environments within microparticles.

Conclusions:

• Various approaches can mitigate protein destabilization and incomplete release from PLGA microparticles.
• Careful consideration of experimental conditions is crucial for accurate in vitro release studies.
• Effective strategies are essential for the successful delivery of therapeutic proteins via microparticle systems.