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Granulocyte collection
R G Strauss1, P A Rohret, M J Randels
1DeGowin Blood Center, University of Iowa Hospitals and Clinics, Iowa City.
Journal of Clinical Apheresis
|January 1, 1991
Summary
Collecting granulocytes for transfusion is optimized using continuous-flow centrifugation leukapheresis. This method, involving steroid-stimulated donors and pentastarch, yields high-quality granulocyte concentrates with mild donor reactions.
Area of Science:
- Hematology
- Transfusion Medicine
- Cellular Therapy
Background:
- Granulocyte transfusions are critical for neutropenic patients and neonates.
- Current methods for granulocyte collection impact concentrate quality and donor safety.
Purpose of the Study:
- To describe optimized techniques for collecting granulocytes for transfusion.
- To evaluate the efficacy and safety of continuous-flow centrifugation leukapheresis for granulocyte collection.
Main Methods:
- Utilized continuous-flow centrifugation leukapheresis.
- Employed steroid-stimulated donors.
- Incorporated pentastarch during the procedure.
Main Results:
- Prepared high-quality granulocyte concentrates.
- Observed mild donor reactions.
- Donor reactions were comparable to automated plateletpheresis.
Conclusions:
- Continuous-flow centrifugation leukapheresis is an effective method for granulocyte collection.
- The technique is well-tolerated by donors.
- This method provides optimal granulocyte concentrates for transfusion support.