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Updated: Jul 8, 2026
05:19
Imaging Flow Cytometry to Study Microbial Autoaggregation
Published on: September 29, 2023
Automated classification of bacterial particles in flow by multiangle scatter measurement and support vector machine
Bartek Rajwa1, Murugesan Venkatapathi, Kathy Ragheb
1Purdue University Cytometry Laboratories, Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA. brajwa@purdue.edu
Abstract:
Biological microparticles, including bacteria, scatter light in all directions when illuminated. The complex scatter pattern is dependent on particle size, shape, refraction index, density, and morphology. Commercial flow cytometers allow measurement of scattered light intensity at forward and perpendicular (side) angles (2 degrees
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01:23Flow Cytometry
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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01:28Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...