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Gene expression in women conceiving spontaneously over the age of 45 years

Yuval Gielchinsky¹, Yoel Bogoch, Gideon Rechavi

¹Department of Obstetrics and Gynecology, Hadassah University Medical Center, Jerusalem, Israel.

Fertility and Sterility

|January 1, 2008

Abstract

Objective:

To examine gene expression profiles of women conceiving spontaneously after the age of 45.

Design:

Expression profiling by complementary DNA microarray analysis.

Setting:

University departments.

Patient(S):

Eight women 45 years or older (study group) who conceived spontaneously and were 6 months after their last delivery were compared with six women aged 45 years old (control group) who had their last delivery before the age of 30.

Intervention(S):

Blood samples were collected from each woman for RNA isolation from peripheral blood mononuclear cells (PBMC).

Main Outcome Measure(S):

Expression profiles of PBMC isolated from each woman were determined by using Affymetrix DNA microarray analysis covering about 15,000 identified genes.

Result(S):

Microarray of global gene expression revealed 671 genes that showed statistically significant differential expression between the study and control groups: 383 genes were overexpressed and 288 were underexpressed. The most significant functional groups defining these genes were: apoptosis, ubiquitination, and energy production. As many as 60 genes also participated in ovarian physiology.

Conclusion(S):

These observations suggest that extended fertility is associated with a unique ability to enrich cellular processes, leading to delayed ovarian senescence.

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