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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Structural insights into the exon junction complex.

Hervé Le Hir1, Gregers Rom Andersen

  • 1Equipe Labélisée La Ligue, Centre de Génétique Moléculaire, associé aux Universités Paris 6 et Paris 11, CNRS UPR2167, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Current Opinion in Structural Biology
|January 1, 2008
PubMed
Summary
This summary is machine-generated.

The exon junction complex (EJC) remains stable on mRNA due to the ATPase activity of eIF4AIII. Structural studies reveal how this complex functions and interacts with other proteins in mRNA metabolism.

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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The exon junction complex (EJC) is a multiprotein complex deposited on spliced mRNAs in higher eukaryotes.
  • The EJC plays crucial roles in mRNA metabolism, including nuclear export, localization, and translation.
  • The core of the EJC includes the DEAD-box protein eIF4AIII, essential for its stability.

Purpose of the Study:

  • To investigate the structural basis of EJC stability and function.
  • To understand the role of eIF4AIII ATPase activity in EJC's long-term binding to mRNA.
  • To identify surface regions on the EJC involved in binding peripheral factors.

Main Methods:

  • X-ray crystallography of the exon junction complex and eIF4AIII.
  • Structural comparison with other DEAD-box proteins bound to RNA and ATP.

Main Results:

  • Provided structural framework for understanding eIF4AIII ATPase function within the EJC.
  • Identified potential surface patches for peripheral factor interactions.
  • Proposed general principles for DEAD-box protein ATPase and RNA remodeling activities based on atomic structures.

Conclusions:

  • The ATPase activity of eIF4AIII is central to the stable association of the EJC with mRNA.
  • Structural insights facilitate further research into EJC-mediated mRNA metabolism.
  • Generalizable principles for DEAD-box protein function can be derived from these structural studies.