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Scotin: A new p63 target gene expressed during epidermal differentiation
Loredana Zocchi1, Jean Christophe Bourdon, Andrea Codispoti
1IDI-IRCCS Biochemistry Laboratory, c/o University of Tor Vergata, Department of Experimental Medicine, Via Montpellier 1, 00133 Rome, Italy.
Biochemical and Biophysical Research Communications
|January 1, 2008
Summary
The study identifies Scotin as a novel target gene of TAp63, a protein crucial for epithelial differentiation. Scotin is upregulated during this process and in response to endoplasmic reticulum stress.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- The p63 protein, a p53 family member, exists in two forms: TAp63 and DeltaNp63.
- Scotin was previously identified as a p53-inducible proapoptotic gene localized to the endoplasmic reticulum and nuclear membrane.
Purpose of the Study:
- To identify and characterize new target genes of TAp63 involved in epithelial differentiation.
- To investigate the role of Scotin in epithelial differentiation and its relationship with TAp63 and endoplasmic reticulum (ER) stress.
Main Methods:
- Analysis of TAp63-induced gene expression during epithelial differentiation.
- Detection of Scotin expression in primary keratinocyte cell lines and human epidermis.
- Investigation of Scotin regulation in response to ER stress.
Main Results:
- Scotin is identified as a novel TAp63 target gene.
- Scotin expression is upregulated in primary keratinocytes undergoing differentiation.
- Scotin is expressed in the suprabasal layer of the epidermis, correlating with TAp63 but not DeltaNp63 expression.
- Scotin expression is induced by ER stress in a p53-dependent or independent manner.
Conclusions:
- Scotin is a newly discovered TAp63 target gene induced during epithelial differentiation.
- Epithelial differentiation involves ER stress induction, and Scotin plays a role in this process.
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