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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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Enumeration of salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk

Burkhard Malorny1, Charlotta Löfström, Martin Wagner

  • 1Federal Institute for Risk Assessment, National Salmonella Reference Laboratory, Diedersdorfer Weg 1, D-12277 Berlin, Germany. burkhard.malorny@bfr.bund.de

Applied and Environmental Microbiology
|January 1, 2008
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...