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Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Published on: July 4, 2007
Characterization of a dengue patient cohort in Recife, Brazil
Marli Tenório Cordeiro1, Ana Maria Silva, Carlos A A Brito
1Laboratório de Virologia e Terapia Experimental do Centro de Pesquisas Aggeu Magalhães-CPqAM, Fiocruz, Recife, Pernambuco, Brazil.
Abstract:
From 2004 to 2006, 658 patients with suspected dengue virus infections were enrolled in a clinical dengue cohort established in Recife, Pernambuco, located at the northeastern region of Brazil. A total of 2,364 blood samples were collected, and serum, plasma, and cells were cryopreserved. Among the suspected cases, 354 (54%) were confirmed as acute DENV-3 infection based on reverse transcription-polymerase chain reaction, virus isolation, and ELISA-IgM. According to WHO criteria, 29.4% of the positive acute cases were classified as dengue fever (DF) and 8.2% of the cases as dengue hemorrhagic fever (DHF), grade 1 or 2. The DHF cases represent 100% of those confirmed in Recife during the period of the study. The dengue cases that did not fulfill the definition of either DHF or DF were classified as DF complicated and accounted for 44.0% of the cases. All the acute cases were classified as either primary or secondary acute dengue virus infections. Secondary infection was predominant in patients with DF; however, there was no predominance of either primary or secondary infections in patients with DHF.
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