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Polyhydroxybutyrate accumulation by a Serratia sp.

Harriet Lugg¹, Rachel L Sammons, Peter M Marquis

¹School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham, UK.

Biotechnology Letters

|January 1, 2008

Summary

This summary is machine-generated.

Serratia sp. bacteria produce polyhydroxybutyrate (PHB) inclusion bodies under specific nutrient conditions. Optimizing yield is possible, with potential co-production of valuable hydroxyapatite (HA).

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Area of Science:

Microbiology
Biochemistry
Biotechnology

Background:

Continuous culture under carbon limitation can induce specific metabolic pathways in bacteria.
Certain bacteria can accumulate intracellular storage compounds under nutrient-limiting conditions.

Purpose of the Study:

To investigate the formation and characteristics of intracellular inclusion bodies in Serratia sp.
To identify the composition of these inclusion bodies and assess their potential for biopolymer production.
To evaluate the co-production of other valuable materials.

Main Methods:

Cultivation of Serratia sp. under specific carbon and nitrogen conditions.
Citrate and glycerol 2-phosphate utilization assays.
Staining with Sudan Black and Nile Red (NR).
Gas Chromatography (GC) and Fourier-transform infrared (FTIR) spectroscopy for material analysis.
Multi-parameter flow cytometry and electron microscopy for cell analysis.

Main Results:

Serratia sp. formed intracellular electron-transparent inclusion bodies when incubated with citrate and glycerol 2-phosphate without a nitrogen source.
The inclusion bodies were identified as polyhydroxybutyrate (PHB) through chemical analysis.
A maximum yield of 55% stored material per gram of biomass dry weight was calculated.
Flow cytometry and electron microscopy indicated variability in PHB accumulation among cells.
Nanoscale hydroxyapatite (HA) was co-produced, suggesting potential for high-value applications.

Conclusions:

Serratia sp. can be induced to accumulate significant amounts of polyhydroxybutyrate (PHB).
Optimization of culture conditions may enhance PHB yield per cell.
Co-production of nanoscale hydroxyapatite (HA) presents an economically attractive avenue for biomaterial development.