Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Time-resolved proteomics reveals five modules of stress granule disassembly and identifies SYNCRIP as a late-phase clearance factor.

The international journal of biochemistry & cell biology·2026

Same author

Recent trends in dual-acting hybrid antibiotics and combination therapies against Gram-negative pathogens.

Journal of microbiology (Seoul, Korea)·2026

Same author

Cleaved SREBP-2 C-terminal fragment noncanonically drives interleukin-1 receptor-associated kinase 1-dependent apoptosis.

Signal transduction and targeted therapy·2026

Same author

Endotrophin- and CD44-Mediated Heterotypic Signaling Mediates Tumor-Stroma Cross-talk and Facilitates Malignant Progression in Hepatocellular Carcinoma.

Cancer research·2026

Same author

Proximity labeling unveils potential roles of the Miro2-CISD1 network in mitochondrial dynamics and neuronal differentiation.

Communications biology·2026

Same author

Neuronal mitochondrial disaggregase CLPB ameliorates Huntington's disease pathology in mice.

Theranostics·2025

Search research articles

Related Experiment Video

Updated: Jul 8, 2026

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp.

Hyun-Woo Rhee¹, Chang-Ro Lee, Seung-Hyon Cho

¹Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.

Journal of the American Chemical Society

|January 2, 2008

Summary

Researchers created a novel fluorescent sensor, PyDPA, for detecting (p)ppGpp alarmones in bacteria and plants. This tool enables real-time monitoring of (p)ppGpp synthesis, crucial for understanding cellular stress responses.

More Related Videos

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Related Experiment Videos

Last Updated: Jul 8, 2026

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Area of Science:

Biochemistry
Molecular Biology
Chemical Sensing

Background:

The stringent response alarmone guanosine tetra- and pentaphosphate ((p)ppGpp) is vital for bacterial and plant survival.
Accurate detection of (p)ppGpp is essential for studying cellular stress responses and microbial physiology.

Purpose of the Study:

To develop the first selective fluorescent chemosensor for (p)ppGpp.
To enable real-time detection of (p)ppGpp synthesis in vitro.

Main Methods:

Development of a pyrene-excimer based fluorescent chemosensor named PyDPA.
Utilizing fluorescence spectroscopy for selective nucleotide detection in aqueous solutions.
Application of the sensor for monitoring bacterial ribosomal complex activity.

Main Results:

PyDPA demonstrated high selectivity for (p)ppGpp over other common nucleotides in water.
The sensor facilitated real-time detection of in vitro (p)ppGpp synthesis.
Successful application in monitoring bacterial ribosomal complex-mediated synthesis.

Conclusions:

PyDPA is a highly selective and sensitive fluorescent probe for (p)ppGpp.
The developed sensor provides a valuable tool for studying the stringent response in biological systems.
Enables real-time biochemical assays for (p)ppGpp production.