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Updated: Jul 8, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
Sigma factor SigC is required for heat acclimation of the cyanobacterium Synechocystis sp. strain PCC 6803
Ilona Tuominen1, Maija Pollari, Eneas Aguirre von Wobeser
1Plant Physiology and Molecular Biology, Department of Biology, University of Turku, Turku, Finland.
Abstract:
The role of the primary-like sigma factor SigC was studied in Synechocystis. Under high temperature stress (48 degrees C) the DeltasigC inactivation strain showed a lower survival rate than the control strain. The DeltasigC strain grew poorly at 43 degrees C in liquid cultures under normal air. However, change to 3% CO(2) enhanced growth of DeltasigC at 43 degrees C. Differences in expression of many genes related to the carbon concentrating mechanisms between the control and the DeltasigC strain were recorded with a genome-wide DNA microarray. We suggest that low solubility of CO2 at high temperature is one of the factors contributing to the poor thermotolerance of the DeltasigC strain.
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