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Updated: Jun 29, 2026

14:53
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
[Quantitative photometric analysis of ethanol using purified alcohol oxidase and mutant cells of methylotrophic
Abstract:
The photometric method is suggested for ethanol analysis by means of purified alcohol oxidase isolated from a wild strain of methylotrophic yeast Hansenula polymorpha. Three different chromogenic systems for peroxidative oxidation together with alcohol oxidase reaction have been used. For the first time the intact cells of the catalase-negative mutant as a source of alcohol oxidase are employed in the photometric alcohol assay. The prospects for application of the latter method for practical purposes are discussed.
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