[In vitro culture of pulp cells]

The purpose of our study was the culture of cellular elements of the pulp. Migration and proliferation of fibroblast-like cells can be observed during the in vitro culture of pulpal tissue. However, no mitotic acticity of the odontoblasts could be induced by nutritive variations of the culture medium. The proliferation of the fibroblastlike cells varied during the primary culture and the first subculture. By contrast, during the second subculture there is a homogeneous process of growth. The growth rate of the second subcultures is therefore suitable for a biological evaluation of dental materials.