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Published on: October 31, 2019
Impact of an oil-based lubricant on the effectiveness of the sterilization processes
William A Rutala1, Maria F Gergen, David J Weber
1Department of Hospital Epidemiology, University of North Carolina Health Care System, Chapel Hill, North Carolina 27599-7030, USA. brutala@unch.unc.edu
Abstract:
Surgical instruments, including hinged instruments, were inoculated with test microorganisms (ie, methicillin-resistant Staphylococcus aureus, approximately 2 x 10(6) colony-forming units [cfu]; Pseudomonas aeruginosa, approximately 3 x 10(6) cfu; Escherichia coli, approximately 2 x 10(5) cfu; vancomycin-resistant enterococci, 1 x 10(5) cfu; Geobacillus stearothermophilus spores, 2 x 10(5) cfu or more; or Bacillus atrophaeus spores, 9 x 10(4) cfu or more), coated with an oil-based lubricant (hydraulic fluid), subjected to a sterilization process, and then samples from the instruments were cultured. We found that the oil-based lubricant did not alter the effectiveness of the sterilization process because high numbers of clinically relevant bacteria and standard test spores (which are relatively resistant to the sterilization process) were inactivated.
Insights
An oil-based lubricant used on surgical instruments did not hinder sterilization effectiveness. High levels of bacteria and resistant spores were inactivated, ensuring instrument safety.
Area of Science:
- Microbiology
- Sterilization Science
- Surgical Technology
Background:
- Effective sterilization of surgical instruments is critical for preventing healthcare-associated infections.
- Lubricants are often used on surgical instruments, particularly hinged ones, but their impact on sterilization efficacy requires evaluation.
- Concerns exist regarding potential interference of lubricants with sterilization processes.
Purpose of the Study:
- To determine if an oil-based lubricant affects the efficacy of a standard sterilization process for surgical instruments.
- To assess the inactivation of common and resistant microorganisms when instruments are lubricated before sterilization.
Main Methods:
- Surgical instruments, including hinged types, were contaminated with various test microorganisms (bacteria and spores).
- Instruments were coated with an oil-based lubricant (hydraulic fluid).
- Lubricated instruments underwent a sterilization process, followed by microbial culturing to assess inactivation.
Main Results:
- The oil-based lubricant did not compromise the sterilization process's effectiveness.
- High concentrations of clinically relevant bacteria, including methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa, were inactivated.
- Standard test spores, such as Geobacillus stearothermophilus and Bacillus atrophaeus, which are resistant to sterilization, were also effectively inactivated.
Conclusions:
- Oil-based lubricants, like hydraulic fluid, can be used on surgical instruments without negatively impacting sterilization efficacy.
- The study confirms that standard sterilization methods remain effective in eliminating microbial contamination even in the presence of this lubricant.
- Findings support the continued use of lubricants for surgical instrument maintenance while ensuring patient safety through effective sterilization.
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