Related Experiment Video
Updated: Jul 8, 2026

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Published on: February 21, 2019
Rab proteins in endocytosis and Glut4 trafficking
V Kaddai1, Y Le Marchand-Brustel, M Cormont
1Institut National de la Santé et de la Recherche Médicale INSERM U568 Faculté de Médecine, Université de Nice-Sophia Antipolis, Nice Cedex, France.
Abstract:
The intracellular trafficking of numerous proteins requires a tight control to fulfil their physiological functions. It is the case of the adipocyte and muscle glucose transporter Glut4 that is retained intracellularly until insulin induces its recruitment to the plasma membrane. Rabs are evolutionarily conserved small GTPases that control intracellular traffic events from yeast to mammalian cells. In the past few decades, considerable progresses have been made in identifying the route of Glut4, the Rabs involved in controlling it, and more recently the connection between insulin signalling and Glut4 trafficking through Rab activity control.
Related Concept Videos
Rab Cascades
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Recycling Endosomes and Transcytosis
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
Clathrin Coated Vesicles
Export of Misfolded Proteins out of the ER
The Early Endosome: Endocytosis of Transferrin

