Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electromagnetic Waves in Matter01:30

Electromagnetic Waves in Matter

Electromagnetic waves can travel in the vacuum as well as in matter. For example light, which is an electromagnetic wave, can travel through air, water, or glass.
Consider the electromagnetic wave passing through a dielectric medium. In such a case, Maxwell's equations get modified. In Ampere's law, ε0 , the dielectric permittivity of free space is replaced with ε, the permittivity of dielectric. Also, the vacuum permeability μ0 is replaced by the permeability of the medium, μ.
Furthermore, the...
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of Adaptive Behavior and Wandering with Serious Injuries in Young Children with Autism: Study to Explore Early Development.

Journal of autism and developmental disorders·2025
Same author

Floating offshore wind potential for Mediterranean countries.

Heliyon·2024
Same author

Perspectives on weak interactions in complex materials at different length scales.

Physical chemistry chemical physics : PCCP·2023
Same author

The use of surfactant-filled mesoporous silica as an immobilising medium for a fluorescence lifetime pH indicator, providing long-term calibration stability.

RSC advances·2022
Same author

Temperature-Dependent Bending Rigidity of AB-Stacked Bilayer Graphene.

Physical review letters·2022
Same author

Prominence of Terahertz Acoustic Surface Plasmon Excitation in Gas-Surface Interaction with Metals.

The journal of physical chemistry letters·2021

Related Experiment Video

Updated: Jul 8, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Imaging with neutral atoms: a new matter-wave microscope.

M Koch1, S Rehbein, G Schmahl

  • 1Graz University of Technology, Institute of Experimental Physics, Petersgasse 16, 8010, Graz, Austria.

Journal of Microscopy
|January 5, 2008
PubMed
Summary

Researchers developed a new matter-wave microscopy technique using a focused beam of helium atoms. This innovation produced the first 2D images with this atomic beam microscopy, advancing imaging capabilities.

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

Related Experiment Videos

Last Updated: Jul 8, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

Area of Science:

  • Atomic physics
  • Microscopy
  • Materials science

Background:

  • Matter-wave microscopy, pioneered with electron beams in 1932, has evolved significantly.
  • Previous techniques relied on charged particles, limiting certain applications.

Purpose of the Study:

  • To introduce a novel matter-wave microscopy instrument utilizing a focused beam of neutral ground-state helium atoms.
  • To demonstrate the capability of this new technique by acquiring the first 2D images.

Main Methods:

  • A focused beam of helium atoms was scanned across a free-standing hexagonal copper grating.
  • Transmission mode imaging was employed using a focused beam with a minimum spot size of 2.0 micrometers.
  • A zone plate was used for focusing the atomic beam, achieving a smallest focus of 1.9 +/- 0.1 micrometers.

Main Results:

  • The first 2D images were successfully obtained using neutral helium atom beam microscopy.
  • The experiment imaged a hexagonal copper grating with a period of approximately 36 micrometers.
  • The achieved resolution was limited by the atomic beam's speed ratio and the zone plate's chromatic aberrations.

Conclusions:

  • This study presents a significant advancement in matter-wave microscopy by successfully employing neutral helium atoms for imaging.
  • The developed instrument and technique demonstrate the potential for new applications in high-resolution imaging.
  • Future improvements in source and focusing optics are expected to further enhance resolution, approaching theoretical limits set by the particle's wavelength.