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Updated: Feb 5, 2026

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Mechanistic studies of fluorescent sensors for the detection of reactive oxygen species
Belinda Heyne1, Sara Ahmed, J C Scaiano
1University of Ottawa, Department of Chemistry, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada.
Abstract:
Two new sensors for the detection of reactive oxygen species have been synthesized and characterized; they contain the 4-amino-7-nitrobenzofurazan (ABF) moiety covalently tethered to a phenol. Comparison of sensors ABFhd and dABFhd (the latter containing two ortho-methyl groups) shows that introduction of steric bulk leads to an improvement of fluorescent sensor performance, thus confirming our previous predictions based on computational chemistry. ABFhd and dABFhd are new tools for biological applications involving reactive oxygen species, in particular oxygen-centered radicals.
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