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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: Jul 8, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

[A study on miRNA alternation after H2O2-induced PC12 cell apoptosis using microarray technique].

Fen Wang¹, Zhao-hui Li

¹Department of Forensic Medicine, Zhongshan school of Medicine, Sun Yat-sen University, Guangzhou 510080, China. wangfen_2004@163.com

Fa Yi Xue Za Zhi

|January 8, 2008

Summary

This study identified six significantly down-regulated microRNAs (miRNAs) in PC12 cells undergoing hydrogen peroxide (H2O2)-induced apoptosis. These findings may offer new insights into the pathogenesis of nerve cell apoptosis.

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Last Updated: Jul 8, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Context:

Oxidative stress is implicated in neuronal apoptosis.
Hydrogen peroxide (H2O2) is a common inducer of oxidative stress in cellular models.
PC12 cells are a widely used cell line for studying neuronal function and apoptosis.

Purpose:

To identify differentially expressed microRNAs (miRNAs) in PC12 cells experiencing H2O2-induced apoptosis compared to normal PC12 cells.
To utilize microarray technology for comprehensive miRNA profiling.

Summary:

PC12 cells were exposed to varying concentrations of H2O2, and cell viability and apoptosis rates were assessed.
Microarray analysis revealed the expression profiles of miRNAs in both control and H2O2-treated groups.
Six miRNAs were found to be significantly downregulated in H2O2-induced apoptotic PC12 cells.

Impact:

The identified miRNAs may serve as potential biomarkers for H2O2-induced neuronal apoptosis.
These preliminary findings could pave the way for further research into the mechanisms and therapeutic strategies for neurodegenerative conditions.
Understanding miRNA dysregulation in apoptosis contributes to the broader field of neuroprotection research.