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A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
Published on: September 23, 2014
Assessing expression of apoptotic markers using large cohort tissue microarrays
Elah Pick1, Mary M McCarthy, Harriet M Kluger
1Department of Medicine, Yale University School of Medicine, New Haven, CT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 8, 2008
Summary
This study explores apoptotic markers using immunohistochemistry (IHC) on tissue microarrays (TMAs). Automated analysis quantifies protein expression, revealing cellular apoptotic tendencies in various tissues.
Area of Science:
- Cellular biology
- Biochemistry
- Pathology
Background:
- Apoptosis involves intrinsic and extrinsic pathways with pro- and anti-apoptotic proteins.
- Immunohistochemistry (IHC) assesses protein expression, indicating cellular apoptotic tendencies.
- Tissue microarray (TMA) technology enables high-throughput analysis of numerous patient specimens.
Purpose of the Study:
- To discuss TMA construction and staining methods.
- To present examples of apoptotic marker assessment in malignant and benign cells.
- To introduce a novel automated method for quantitative in situ protein expression analysis.
Main Methods:
- Tissue microarray (TMA) construction and staining protocols.
- Immunohistochemistry (IHC) for apoptotic marker detection.
- Automated, quantitative analysis of in situ protein expression.
Main Results:
- Demonstration of TMA utility in evaluating large specimen cohorts.
- Quantitative assessment of apoptotic marker expression in diverse cell types.
- Validation of automated analysis for reliable protein expression quantification.
Conclusions:
- TMA technology combined with IHC provides a powerful approach for studying apoptosis.
- Automated quantitative analysis enhances the accuracy and efficiency of assessing apoptotic markers.
- This methodology facilitates a deeper understanding of apoptotic regulation in health and disease.
