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Related Concept Videos

Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.

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Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

Caspase activity assays.

Andrew L Niles1, Richard A Moravec, Terry L Riss

  • 1Promega Corporation, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 8, 2008
PubMed
Summary

Caspase activity assays using advanced chemistries offer sensitive, quantifiable measurements of apoptosis. These cost-efficient methods rapidly screen numerous conditions for drug discovery and biological research.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Apoptosis is a critical biological process.
  • Caspase activity is a key indicator of apoptosis.
  • Accurate measurement of caspase activity is essential for research.

Purpose of the Study:

  • To introduce advanced caspase activity assays.
  • To highlight their sensitivity and efficiency.
  • To demonstrate their utility in exploring apoptotic responses.

Main Methods:

  • Utilizing multi-well plate formats.
  • Employing novel fluorescent and luminescent chemistries.
  • Implementing "add-mix-measure" protocols for direct sample analysis.

Main Results:

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  • Achieved unprecedented sensitivity in caspase activity determination.
  • Enabled quantifiable and definitive measures of caspase induction or inhibition.
  • Demonstrated cost-efficiency and rapid screening capabilities.

Conclusions:

  • Advanced caspase assays provide powerful tools for apoptosis research.
  • These methods facilitate the rapid exploration of numerous experimental conditions.
  • They offer a sensitive, quantifiable, and efficient approach to studying apoptosis modulation.