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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.

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Related Experiment Video

Updated: Jul 8, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

Multiplex caspase activity and cytotoxicity assays.

Andrew L Niles¹, Richard A Moravec, Terry L Riss

¹Promega Corporation, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 8, 2008

Summary

Multiplexed assays enable multiple cellular measurements in one well, enhancing cost-efficiency and data richness. Combining caspase activity with viability assays provides normalized, mechanistic insights into compound effects.

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Last Updated: Jul 8, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

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Evaluation of Caspase Activation to Assess Innate Immune Cell Death

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Published on: January 20, 2023

Area of Science:

Cellular biology
Biochemistry
Assay development

Background:

Multiplexed assay chemistries allow for multiple cellular parameter measurements within a single assay well.
This approach offers enhanced cost-efficiency and richer informational content regarding compounds or treatments.
Parallel assays lack the normalization capabilities inherent in combined measurements.

Purpose of the Study:

To highlight the advantages of multiplexed assay chemistries in cellular research.
To demonstrate the utility of multiplexed caspase activity assays for evaluating compound effects.
To show how combining assays aids in mechanistic and normalization strategies.

Main Methods:

Utilizing multiplexed assay chemistries for simultaneous measurements.
Performing multiplexed caspase activity assays to assess initiator and effector caspase induction.
Integrating caspase activity assays with viability and/or cytotoxicity assays within the same sample.

Main Results:

Multiplexed assays provide more informational content compared to parallel assays.
Combined activity profiles within a single sample allow for intrinsic normalization.
Caspace activity assays multiplexed with viability/cytotoxicity assays support mechanistic conclusions.

Conclusions:

Multiplexed assays are a cost-efficient and informative experimental practice.
The integration of different assay types within a single well enhances data normalization and mechanistic understanding.
This approach is valuable for characterizing the effects of candidate compounds and treatments on cellular processes.