Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GC level and expression of human coding sequences.

Stilianos Arhondakis1, Oliver Clay, Giorgio Bernardi

  • 1Laboratory of Molecular Evolution, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy.

Biochemical and Biophysical Research Communications
|January 8, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FieldNA: a 3D printed vertical microfluidic device for portable nucleic acid isolation from olive oil samples.

Frontiers in bioengineering and biotechnology·2025
Same author

A Miniaturized System for Rapid, Isothermal Detection of SARS-CoV-2 in Human and Environmental Samples.

Biomedicines·2023
Same author

The "Genomic Code": DNA Pervasively Moulds Chromatin Structures Leaving no Room for "Junk".

Life (Basel, Switzerland)·2021
Same author

Hybrid Aptamer-Molecularly Imprinted Polymer (aptaMIP) Nanoparticles from Protein Recognition-A Trypsin Model.

Macromolecular bioscience·2021
Same author

IsoXpressor: A Tool to Assess Transcriptional Activity within Isochores.

Genome biology and evolution·2020
Same author

The Genomic Code: A Pervasive Encoding/Molding of Chromatin Structures and a Solution of the "Non-Coding DNA" Mystery.

BioEssays : news and reviews in molecular, cellular and developmental biology·2019
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
Same journal

Development of a vitamin-related gene signature to predict the immune characteristics and prognosis of glioma.

Biochemical and biophysical research communications·2026
Same journal

Cyclo(Ser-Tyr) L/D spatial isomerism exerts opposing effects on benign prostatic hyperplasia.

Biochemical and biophysical research communications·2026
Same journal

Necrotic tumor cells contribute to pro-tumoral responses and inflammatory mediator production via NF-κB activation in oral squamous cell carcinoma.

Biochemical and biophysical research communications·2026
Same journal

GPR4 promotes ferroptosis in nucleus pulposus cells via MAPK-dependent mitophagy suppression.

Biochemical and biophysical research communications·2026
See all related articles

GC content in the third codon position (GC(3)) influences gene expression. While GC(3)-rich genes show higher expression, plasmid context composition also significantly impacts results.

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Expression Regulation

Background:

  • GC-rich genes generally exhibit higher expression levels in mammals.
  • Previous studies suggested GC(3) intrinsically boosts gene expression efficiency.

Purpose of the Study:

  • To investigate the influence of GC content, specifically GC(3), on gene expression.
  • To determine if GC(3) intrinsically enhances expression or if other factors are involved.

Main Methods:

  • Comparing expression levels of GC(3)-rich and GC(3)-poor gene versions inserted into vector plasmids.
  • Utilizing transfection experiments to assess gene expression efficiency.

Main Results:

  • GC(3)-rich genes were expressed more efficiently than GC(3)-poor genes in plasmid vectors.

Related Experiment Videos

  • The study identified that plasmid context compositional properties significantly affected expression results.

    • Conclusions:

    • While GC(3) appears to enhance expression, the surrounding plasmid context plays a crucial role and can influence observed expression levels.
    • Eliminating genomic context does not fully isolate the effect of GC(3) due to the introduction of plasmid context.