Unveiling substrate RNA binding to H/ACA RNPs: one side fits all
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Updated: Jul 8, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
1Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA. hongli@sb.fsu.edu
Abstract:
The H/ACA RNP pseudouridylases function on a large number of extraordinarily complex RNA substrates including pre-ribosomal and small nuclear RNAs. Recent structural data show that H/ACA RNPs capture their RNA substrates via a simple one-sided attachment model. However, the precise placement of each RNA substrate into the active site of the catalytic subunit relies on the essential functions of the RNP proteins. The specific roles of each H/ACA RNP protein are being elucidated by a combination of structural and biochemical studies.
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