Related Experiment Video
Updated: Jul 8, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Theoretical study of sequence-dependent nanopore unzipping of DNA
1Nanobiophysique, Ecole Superieure Physique et Chimie Industrielles, Centre National de Recherche Scientifique, Paris, France. ulrich.bockelmann@espci.fr
Abstract:
We theoretically investigate the unzipping of DNA electrically driven through a nanometer-size pore. Taking the DNA base sequence explicitly into account, the unpairing and translocation process is described by a biased random walk in a one-dimensional energy landscape determined by the sequential basepair opening. Distributions of translocation times are numerically calculated as a function of applied voltage and temperature. We show that varying these two parameters changes the dynamics from a predominantly diffusive behavior to a dynamics governed by jumps over local energy barriers. The work suggests experimentally studying sequence effects, by comparing the average value and standard deviation of the statistical distribution of translocation times.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Lagging Strand Synthesis
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Sanger Sequencing
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
The DNA Helix

