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Related Experiment Video

Updated: Jul 8, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

Clara cell adhesion and migration to extracellular matrix.

Jeffrey J Atkinson¹, Tracy L Adair-Kirk, Diane G Kelley

¹Department of Internal Medicine, Pulmonary and Critical Care Division, Washington University School of Medicine, St, Louis, MO, USA. jjatkins@im.wustl.edu

Respiratory Research

|January 9, 2008

Summary

Clara cells, crucial for small airway repair, migrate differently based on matrix. Their movement toward fibronectin and laminin is integrin-dependent, but toward collagen, it is not.

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Last Updated: Jul 8, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

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Published on: October 5, 2014

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

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Published on: April 3, 2015

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Area of Science:

Pulmonary Biology
Cellular Biology
Extracellular Matrix Research

Background:

Clara cells are the primary epithelial progenitor cells in the small airways, a critical region implicated in various lung diseases.
While epithelial cell migration in the lungs is studied, the specific migratory behavior of Clara cells remains underexplored.

Purpose of the Study:

To investigate the migratory response of mouse Clara cells and a Clara-like cell line (C22) towards extracellular matrix gradients.
To determine the integrin dependence of Clara cell migration on different matrix substrates.

Main Methods:

Modified Clara cell isolation techniques were employed to obtain primary mouse Clara cells and the C22 cell line.
Adhesion and migration assays were performed using matrix substrate gradients, including fibronectin, type I collagen, and laminin 511.
Integrin dependence was assessed using RGD-containing peptides to block integrin-mediated signaling.

Main Results:

Clara cells demonstrated preferential adhesion to fibronectin and type I collagen.
Clara cell migration exhibited haptotaxis, responding to gradients of matrix substrates.
Migration of C22 cells towards fibronectin and laminin 511 was significantly dependent on RGD-binding integrins, whereas migration towards type I collagen was RGD-independent.

Conclusions:

Clara cells exhibit integrin-mediated migration in response to extracellular matrix components, similar to alveolar type II and ciliated bronchiolar epithelial cells.
These findings highlight distinct integrin-receptor interactions for different matrix substrates, suggesting a complex migratory mechanism for Clara cells.
The migratory plasticity of Clara cells is relevant to understanding tissue repair processes in injured lung tissues.